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                  [1]汪濤,何琎,劉亞,等.姜黃素在抑制紫外線B誘導的人視網膜色素上皮細胞DNA氧化損傷和凋亡中的作用[J].眼科新進展,2023,43(7):530-535.[doi:10.13389/j.cnki.rao.2023.0107]
                   WANG Tao,HE Jin,LIU Ya,et al.Inhibitory effect of curcumin on ultraviolet-B-induced DNA oxidative damage and apoptosis in human retinal pigment epithelial cells[J].Recent Advances in Ophthalmology,2023,43(7):530-535.[doi:10.13389/j.cnki.rao.2023.0107]
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                  姜黃素在抑制紫外線B誘導的人視網膜色素上皮細胞DNA氧化損傷和凋亡中的作用/HTML
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                  《眼科新進展》[ISSN:1003-5141/CN:41-1105/R]

                  卷:
                  43卷
                  期數:
                  2023年7期
                  頁碼:
                  530-535
                  欄目:
                  實驗研究
                  出版日期:
                  2023-07-05

                  文章信息/Info

                  Title:
                  Inhibitory effect of curcumin on ultraviolet-B-induced DNA oxidative damage and apoptosis in human retinal pigment epithelial cells
                  作者:
                  汪濤何琎劉亞盧萍張夢瑜劉建軍
                  215000 江蘇省蘇州市,蘇州科技城醫院眼科
                  Author(s):
                  WANG TaoHE JinLIU YaLU PingZHANG MengyuLIU Jianjun
                  Department of Ophthalmology,Suzhou Science and Technology Town Hospital,Suzhou 215000,Jiangsu Provience,China
                  關鍵詞:
                  姜黃素紫外線B人視網膜色素上皮細胞DNA氧化損傷細胞凋亡
                  Keywords:
                  curcumin ultraviolet-B human retinal pigment epithelial cell DNA oxidative damage apoptosis
                  分類號:
                  R776
                  DOI:
                  10.13389/j.cnki.rao.2023.0107
                  文獻標志碼:
                  A
                  摘要:
                  目的 探討姜黃素在紫外線B(UVB)誘導的人視網膜色素上皮(RPE)細胞DNA氧化損傷和凋亡中的作用機制。
                  方法 將人視網膜色素上皮細胞株(ARPE-19)隨機分為空白對照組、二甲基亞砜(DMSO)組、姜黃素組、UVB照射組、UVB+DMSO組和UVB+姜黃素組。CCK-8實驗檢測姜黃素對UVB照射前后ARPE-19細胞活力的影響;免疫熒光檢測DNA氧化損傷標志蛋白磷酸化組蛋白H2AX(γH2AX)的定位;Western blot檢測γH2AX、凋亡相關蛋白[B淋巴細胞瘤-2基因(BCL-2)和BCL-2相關X蛋白(BAX)]的表達變化;使用X-rosamine衍生物(Mito-Tracker Red CMXRos)和異硫氰酸熒光素(FITC)標記的鈣離子依賴的磷脂結核蛋白Annexin V(Annexin V-FITC)來檢測UVB誘導的ARPE-19細胞線粒體膜電位變化和細胞凋亡情況。
                  結果 與空白對照組相比,DMSO組DMSO處理24 h后的ARPE-19細胞活力無明顯改變(P>0.05)。與DMSO組相比,姜黃素組采用15 μmol·L-1 姜黃素處理24 h后ARPE-19細胞活力輕度升高(P<0.05),表明15 μmol·L-1姜黃素對ARPE-19細胞無毒性作用,后續實驗采用該濃度處理細胞。與空白對照組相比,UVB照射組ARPE-19細胞活力降低,γH2AX和BAX蛋白表達水平升高,BCL-2蛋白表達水平降低,線粒體膜電位降低,細胞凋亡增加(均為P<0.05)。與UVB照射組相比,UVB+DMSO組ARPE-19細胞活力,γH2AX、BAX以及BCL-2蛋白表達水平均無明顯變化(均為P>0.05),線粒體膜電位和細胞凋亡均無明顯改變(均為P>0.05)。與UVB+DMSO組相比,UVB+姜黃素組ARPE-19細胞活力升高,γH2AX和BAX蛋白表達水平降低,BCL-2蛋白表達水平升高,線粒體膜電位升高,細胞凋亡減少(均為P<0.05)。
                  結論 姜黃素能減輕UVB誘導的RPE細胞DNA氧化損傷和凋亡,有望為姜黃素在年齡相關性黃斑變性中的作用機制研究提供新的思路。
                  Abstract:
                  Objective To investigate the effects of curcumin on DNA oxidative damage and apoptosis in human retinal pigment epithelial (RPE) cells induced by ultraviolet-B (UVB).
                  Methods Human RPE cell lines (ARPE-19) were divided into the blank control (BC) group,dimethyl sulfoxide (DMSO) group,curcumin group,UVB irradiation group,UVB+DMSO group,and UVB+curcumin group.The effect of curcumin on the activity of ARPE-19 cells before and after UVB irradiation was assessed by CCK-8 assay.The H2AX phosphorylation (γH2AX),a marker of DNA oxidative damage,was localized by immunofluorescence.The expression levels of γH2AX and apoptosis-related proteins,including B-cell lymphoma 2 (BCL-2) and Bcl-2-associated X protein (BAX),were measured by Western blot.X-rosamine derivative Mito-Tracker Red CMXRos and Annexin V labeled by fluorescein isothiocyanate (Annexin V-FITC) were used to detect UVB-induced mitochondrial membrane potential changes and apoptosis in ARPE-19 cells.
                  Results Compared with the BC group,ARPE-19 cells in the DMSO group showed no significant changes in viability after being treated with DMSO for 24 h (P>0.05).Compared with the DMSO group,the viability of ARPE-19 cells after being treated with 15 μmol·L-1 curcumin for 24 h in the curcumin group increased slightly (P<0.05),indicating that curcumin at this concentration had no cytotoxicity,and it was used in the subsequent experiments.Compared with the BC group,ARPE-19 cell viability,BCL-2 level,and mitochondrial membrane potential in the UVB irradiation group decreased,while γH2AX and BAX levels and cell apoptosis increased (all P<0.05).Compared with the UVB irradiation group,ARPE-19 cell viability,γH2AX,BAX and BCL-2 levels,mitochondrial membrane potential,and cell apoptosis in the UVB+DMSO group showed no significant changes (all P>0.05).Compared with the UVB+DMSO group,ARPE-19 cell viability,BCL-2 level,and mitochondrial membrane potential increased,while γH2AX and BAX levels and cell apoptosis decreased in the UVB+curcumin group (all P<0.05).
                  Conclusion Curcumin can reduce DNA oxidative damage and apoptosis in RPE cells induced by UVB,which provides a new idea for studying the effects of curcumin on age-related macular degeneration.

                  參考文獻/References:

                  [1] LASHKARI K,TEAGUE G C,BEATTIE U,BETTS J,KUMAR S,MCLAUGHLIN M M,et al.Plasma biomarkers of the amyloid pathway are associated with geographic atrophy secondary to age-related macular degeneration[J].PLoS One,2020,15(8):e0236283.
                  [2] XIA H,ZHANG Q,SHEN Y,BAI Y,MA X,ZHANG B,et al.ube3d,a new gene associated with age-related macular degeneration,induces functional changes in both in vivo and in vitro studies[J].Mol Ther Nucleic Acids,2020,20:217-230.
                  [3] FRITSCHE L G,IGL W,BAILEY J N,GRASSMANN F,SENGUPTA S,BRAGG-GRESHAM J L,et al.A large genome-wide association study of age-related macular degeneration highlights contributions of rare and commonvariants[J].Nat Genet,2016,48(2):134-143.
                  [4] MAHENDRA C K,TAN L T H,PUSPARAJAH P,HTAR T T,CHUAH L H,LEE V S,et al.Detrimental effects of UVB on retinal pigment epithelial cells and its role in age-related macular degeneration[J].Oxid Med Cell Longev,2020,2020:1904178.
                  [5] HE J,LONG C,HUANG Z,ZHOU X,KUANG X,LIU L,et al.PTEN reduced UVB-mediated apoptosis in retinal pigment epithelium cells[J].Biomed Res Int,2017,2017:3681707.
                  [6] LAORODPHUN P,CHERNGWELLING R,PANYA A,ARJINAJARN P.Curcumin protects rats against gentamicin-induced nephrotoxicity by amelioration of oxidative stress,endoplasmic reticulum stress and apoptosis[J].Pharm Biol,2022,60(1):491-500.
                  [7] ZHAO L,LUO R,YU H,LI S,YU Q,WANG W,et al.Curcumin protects human umbilical vein endothelial cells against high oxidized low density lipoprotein-induced lipotoxicity and modulates autophagy[J].Iran J Basic Med Sci,2021,24(12):1734-1742.
                  [8] SIM R H,SIRASANAGANDLA S R,DAS S,TEOH S L.Treatment of glaucoma with natural products and their mechanism of action:an update[J].Nutrients,2022,14(3):534.
                  [9] BUCOLO C,DRAGO F,MAISTO R,ROMANO G L,D’AGATA V,MAUGERI G,et al.Curcumin prevents high glucose damage in retinal pigment epithelial cells through ERK1/2-mediated activation of the Nrf2/HO-1 pathway[J].J Cell Physiol,2019,234(10):17295-17304.
                  [10] 陳月芹,黃振平,程瑩瑩,薛春燕.姜黃素對核轉錄因子-κB表達的影響及小鼠角膜紫外線照射損傷的抑制作用[J].眼科新進展,2017,37(11):1015-1017.
                  CHEN Y Q,HUANG Z P,CHENG Y Y,XUE C Y.Effects of curcumin on NF-κB expression and UVA-induced corneal injury in mice[J].Rec Adv Ophthalmol,2017,37(11):1015-1017.
                  [11] HUANG S,LIU C H,WANG Z,FU Z,BRITTON W R,BLOMFIELD A K,et al.REV-ERBalpha regulates age-related and oxidative stress-induced degeneration in retinal pigment epithelium via NRF2[J].Redox Biol,2022,51:102261.
                  [12] AN M J,KIM C H,NAM G Y,KIM D H,RHEE S,CHO S J,et al.Transcriptome analysis for UVB-induced phototoxicity in mouse retina[J].Environ Toxicol,2018,33(1):52-62.
                  [13] PATTON W P,ROUTLEDGE M N,JONES G D,LEWIS S E,ARCHER D B,DAVIES R J,et al.Retinal pigment epithelial cell DNA is damaged by exposure to benzo[a]pyrene,a constituent of cigarette smoke[J].Exp Eye Res,2002,74(4):513-522.
                  [14] ZHU T,WANG L,WANG L P,WAN Q.Therapeutic targets of neuroprotection and neurorestoration in ischemic stroke: applications for natural compounds from medicinal herbs[J].Biomed Pharmacother,2022,148:112719.
                  [15] BAHRAMI A,SATHYAPALAN T,MOALLEM S A,SAHEBKAR A.Counteracting arsenic toxicity: curcumin to the rescue?[J].J Hazard Mater,2020,400:123160.
                  [16] PARK J H,LEE B M,KIM H S.Potential protective roles of curcumin against cadmium-induced toxicity and oxidative stress[J].J Toxicol Environ Health B Crit Rev,2021,24(3):95-118.
                  [17] DENG H,WAN M,LI H,CHEN Q,LI R,LIANG B,et al.Curcumin protection against ultraviolet-induced photo-damage in Hacat cells by regulating nuclear factor erythroid 2-related factor 2[J].Bioengineered,2021,12(2):9993-10006.
                  [18] LIN C,WU X.Curcumin protects trabecular meshwork cells from oxidative stress[J].Invest Ophthalmol Vis Sci,2016,57(10):4327-4332.
                  [19] ZHOU X,KUANG X,LONG C,LIU W,TANG Y,LIU L,et al.Curcumin inhibits proliferation and epithelial-mesenchymal transition of retinal pigment epithelial cells via multiple pathways[J].Curr Mol Med,2017,17(4):312-319.
                  [20] HE Y,CHEN Z,ZHANG R,QUAN Z,XU Y,HE B,et al.Mitochondrial-targeted antioxidant peptide SS31 prevents RPE cell death under oxidative stress[J].Biomed Res Int,2022,2022:6180349.
                  [21] SABET N S,ATASHBAR S,KHANLOU E M,KAHRIZI F,SALIMI A.Curcumin attenuates bevacizumab-induced toxicity via suppressing oxidative stress and preventing mitochondrial dysfunction in heart mitochondria[J].N-S Arch Pharmacol,2020,393(8):1447-1457.
                  [22] WANG X,ZHANG Y,YANG Y,ZHANG W,LUO L,HAN F,et al.Curcumin pretreatment protects against hypoxia/reoxgenation injury via improvement of mitochondrial function,destabilization of HIF-1alpha and activation of Epac1-Akt pathway in rat bone marrow mesenchymal stem cells[J].Biomed Pharmacother,2019,109:1268-1275.

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                  更新日期/Last Update: 2023-07-05
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